Structure Activity Relationship of selective GABA uptake inhibitors

Author(s)
Stine B. Vogensen, Jørgensen, Lars, Karsten K. Madsen, Andreas Jurik, Nrupa Borkar, Emiliano Rosatelli, Brigitte Nielsen, Gerhard Ecker, Arne Schousboe, Rasmus Praetorius Clausen
Abstract

A series of β-amino acids with lipophilic diaromatic side chain was synthesized and characterized pharmacologically on mouse γ-amino butyric acid (GABA) transporter subtypes mGAT1-4 in order to investigate structure activity relationships (SAR) for mGAT2 (corresponding to hBGT-1). Variation in the lipophilic diaromatic side chain was probed to understand the role of the side chain for activity. This yielded several selective compounds of which the best (1R,2S)-5a was more than 10 fold selective towards other subtypes, although potency was moderate. A docking study was performed to investigate possible binding modes of the compounds in mGAT2 suggesting a binding mode similar to that proposed for Tiagabine in hGAT1. Specific interactions between the transporter and the amino acid part of the ligands may account for a reverted preference towards mGAT2 over mGAT1.

Organisation(s)
External organisation(s)
University of Copenhagen, Università degli Studi di Perugia
Journal
Bioorganic & Medicinal Chemistry
Volume
23
Pages
2480-2488
No. of pages
9
ISSN
0968-0896
DOI
https://doi.org/10.1016/j.bmc.2015.03.060
Publication date
05-2015
Peer reviewed
Yes
Austrian Fields of Science 2012
301207 Pharmaceutical chemistry
Keywords
ASJC Scopus subject areas
Drug Discovery, Molecular Medicine, Molecular Biology, Biochemistry, Clinical Biochemistry, Pharmaceutical Science, Organic Chemistry
Portal url
https://ucrisportal.univie.ac.at/en/publications/fed0baf0-2e97-4ea7-9693-dc90ab6521fd