Development of an in vitro blood-brain barrier model based on immortalized porcine brain microvascular endothelial cells
- Author(s)
- Regina Lauer, Roland Bauer, Birgit Linz, Fritz Pittner, Günter Peschek, Gerhard Ecker, Peter Friedl, Christian Noe
- Abstract
Immortalized porcine brain microvessel endothelial cells (PBMEC/C1-2) were used to develop a model for measurement of blood–brain barrier permeation of central nervous system active drugs. Previous studies showed that a system using C6 astrocyte glioma conditioned medium leads to cell layers with transendothelial electrical resistance values up to 300 Ω cm2 and a permeability coefficient Pe of 3.24 ± 0.14 × 10–4 cm/min for U-[14C]sucrose, which is in good agreement to published values and thus indicates the formation of tight junctions in vitro. However, commercially available inserts for the Transwell® system were not permeable for highly lipophilic compounds, such as diazepam. Systematic studies with different insert showed, that inserts with a pore width of 1 μm proved to be optimal for permeation studies of lipophilic compounds. Permeability studies with a set of three benzodiazepines further supported this finding. © 2003 Elsevier SAS. All rights reserved.
- Organisation(s)
- Department of Biochemistry and Cell Biology, Department of Physical Chemistry
- External organisation(s)
- Johann Wolfgang Goethe-Universität Frankfurt am Main, Julius-Maximilians-Universität Würzburg
- Journal
- Il Farmaco
- Volume
- 59
- Pages
- 133-137
- No. of pages
- 5
- ISSN
- 0014-827X
- DOI
- https://doi.org/10.1016/j.farmac.2003.11.007
- Publication date
- 2004
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 3012 Pharmacy, Pharmacology, Toxicology
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/86458416-e0f2-4b48-95a2-80cf8f1cdaba