An Overview of Cell-Based Assay Platforms for the Solute Carrier Family of Transporters

Vojtech Dvorak, Tabea Wiedmer, Alvaro Ingles-Prieto, Patrick Altermatt, Helena Batoulis, Felix Bärenz, Eckhard Bender, Daniela Digles, Franz Dürrenberger, Laura H. Heitman, Adriaan P. IJzerman, Douglas B. Kell, Stefanie Kickinger, Daniel Körzö, Philipp Leippe, Thomas Licher, Vania Manolova, Riccardo Rizzetto, Francesca Sassone, Lia Scarabottolo, Avner Schlessinger, Vanessa Schneider, Hubert J. Sijben, Anna-Lena Steck, Hanna Sundström, Sara Tremolada, Maria Wilhelm, Marina Wright Muelas, Diana Zindel, Claire M. Steppan, Giulio Superti-Furga

The solute carrier (SLC) superfamily represents the biggest family of transporters with important roles in health and disease. Despite being attractive and druggable targets, the majority of SLCs remains understudied. One major hurdle in research on SLCs is the lack of tools, such as cell-based assays to investigate their biological role and for drug discovery. Another challenge is the disperse and anecdotal information on assay strategies that are suitable for SLCs. This review provides a comprehensive overview of state-of-the-art cellular assay technologies for SLC research and discusses relevant SLC characteristics enabling the choice of an optimal assay technology. The Innovative Medicines Initiative consortium RESOLUTE intends to accelerate research on SLCs by providing the scientific community with high-quality reagents, assay technologies and data sets, and to ultimately unlock SLCs for drug discovery.

Department of Pharmaceutical Sciences
External organisation(s)
Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vifor International AG, Bayer AG, Sanofi-Aventis Deutschland GmbH, Leiden University, University of Liverpool, Technical University of Denmark (DTU), Max-Planck-Institut für medizinische Forschung, Axxam SpA, Icahn School of Medicine at Mount Sinai, New York, Emerging Science & Innovation, Medizinische Universität Wien
Frontiers in Pharmacology
No. of pages
Publication date
Peer reviewed
Austrian Fields of Science 2012
301207 Pharmaceutical chemistry
ASJC Scopus subject areas
Pharmacology (medical), Pharmacology
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